Mucosal vaccine delivery by non-recombinant spores of Bacillus subtilis
نویسندگان
چکیده
منابع مشابه
Mucosal vaccine delivery by non-recombinant spores of Bacillus subtilis
Development of mucosal vaccines strongly relies on an efficient delivery system and, over the years, a variety of approaches based on phages, bacteria or synthetic nanoparticles have been proposed to display and deliver antigens. The spore of Bacillus subtilis displaying heterologous antigens has also been considered as a mucosal vaccine vehicle, and shown able to conjugate some advantages of l...
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Tuberculosis (TB) is the most deadly infectious disease in existence, and the only available vaccine, Bacillus Calmette-Guérin (BCG), is almost a century old and poorly protective. The immunological complexity of TB, coupled with rising resistance to antimicrobial therapies, necessitates a pipeline of diverse novel vaccines. Here, we show that Bacillus subtilis spores can be coated with a fusio...
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Bacillus subtilis spores have been used as safe and heat-resistant antigen delivery vectors. Nonetheless, the oral administration of spores typically induces weak immune responses to the passenger antigens, which may be attributed to the fast transit through the gastrointestinal tract. To overcome this limitation, we have developed B. subtilis spores capable of binding to the gut epithelium by ...
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8 Vaccine Development Laboratory, Department of Microbiology, Institute of Biomedical 9 Sciences, University of São Paulo, São Paulo, Brazil. 10 Vaccine Development Laboratory, Department of Parasitology, Institute of Biomedical 11 Sciences, University of São Paulo, São Paulo, Brazil 12 Cancer Genomics Group, International Centre for Genetic Engineering and 13 Biotechnology (ICGEB), Cape Town 7...
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We developed a novel surface display system based on the use of bacterial spores. A protein of the Bacillus subtilis spore coat, CotB, was found to be located on the spore surface and used as fusion partner to express the 459-amino-acid C-terminal fragment of the tetanus toxin (TTFC). Western, dot blot and fluorescent-activated cell sorting analyses were used to monitor TTFC surface expression ...
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ژورنال
عنوان ژورنال: Microbial Cell Factories
سال: 2014
ISSN: 1475-2859
DOI: 10.1186/s12934-014-0115-2